Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Mir-23b down-regulates the expression of target gene of acetaldehyde dehydrogenase 1a1 and increases the sensitivity of cervical cancer stem cells to cisplatin

Yongbing Tao¹, Fuyun Mao², Weihong Gu², Ling Wu², Jing Guo², Ping Xu²

¹Huai’an Second People’s Hospital and The Affiliated Huaian Hospital of Xuzhou Medical University; ²Department of Gynaecology and Obstetrics, Huai’an Maternal and Child Health Care Center, Huai’an 223002, PR China.

For correspondence:- Ping Xu Email: itgq01@163.com

Accepted: 30 December 2020 Published: 31 January 2021

Citation: Tao Y, Mao F, Gu W, Wu L, Guo J, Xu P. Mir-23b down-regulates the expression of target gene of acetaldehyde dehydrogenase 1a1 and increases the sensitivity of cervical cancer stem cells to cisplatin. Trop J Pharm Res 2021; 20(1):29-34 doi: 10.4314/tjpr.v20i1.5

© 2021 The authors.
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Abstract

Purpose: To study the effect of miR-23b on the expression of the target gene of acetaldehyde dehydrogenase 1A1 (ALDH1A1), and cisplatin (CDDP) susceptibility of cervical carcinoma stem cells.

Methods: Human cervical cancer cell line Hela cells were cultured in vitro, and miR-23b mimic and negative control were transfected into the cells using lipofectamine method. The growth of the two groups of cells was determined using growth curve method, and their proliferation measured using plate clone formation. The influence of treatments on the sensitivity of the cells to CDDP was assayed using MTT method. The mRNA expression of ALDH1A1 in Hela cells was assayed using real-time quantitative polymerase hain reation (PCR), while its protein expression was assayed by Western blot.

Results: The levels of expressions of ALDH1A1 protein and mRNA in the miR-23b overexpression group were significantly lower than those in the control group (p < 0.05). The sensitivities of Hela cells to CDDP in the ALDH1A1 inhibition group and the control group were dose-dependent to some extent, but cell inhibition in ALDH1A1 inhibition group markedly increased, relative to control when the CDDP dose was 0.1 ppc (p < 0.01).

Conclusion: Up-regulating the expression of miR-23b significantly inhibits the growth and proliferation of cervical cancer cells, and increases their sensitivity to CDDP via down-regulation of the expression of the target gene for ALDH1A1. Therefore, during cervical carcinoma treatment, increasing the level of miR-23b may produce a chemotherapeutic effect.

Keywords: MiR-23b, Acetaldehyde dehydrogenase 1A1, Cervical cancer, Cisplatin, Sensitivity